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Actually, network operating system is a bit of a misnomer in that the NOS really runs on computers that are servers placed in the network. Figure 4-3 shows a single10 le server and a single11 print server. In reality, on large networks there could be multiple servers in use. Also, for a small of ce, the functionalities of both the le server and print server can be combined in a single server. Being a client/server application, the responsibility for authentication of clients with the authority to connect to the server depends on the server to verify that clients have the necessary valid security credentials. In larger networks with many clients, that function can be placed in entirely separate servers solely responsible for granting network access as well as the permission levels a user will have while logged into the network. There are networks where the software that is being run on a local PC is actually an application located on the server. An example of this is a word processor program that has a xed number of network licenses. The theory is that not all users would use the program simultaneously, so a company could save some costs by sharing applications over the network. Once all the licenses

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labeling, and drug products and the authority to review production records to assure that no errors have occurred or, if errors have occurred, that they have been fully investigated. The responsibilities of the unit extend to approving or rejecting drug products manufactured, processed, packed, or held by contract manufacturers. The organization must assure that the quality control unit has adequate laboratory facilities for the testing and approval (or rejection) of components, drug product containers, closures, packaging materials, in-process materials, and drug products. In addition to duties associated with the approval of materials and nished products, the unit is also responsible for approving or rejecting all procedures or speci cations impacting on the identity, strength, quality, and purity of the drug product. This includes review and approval of procedures for production and process control, including any changes to these procedures. These procedures, and the responsibilities and procedures applicable to the quality control unit within the organization, must be written and followed. All speci cations, standards, sampling plans, test procedures, or other laboratory control mechanisms, including any changes, must be in writing and reviewed and approved by the quality control unit. Written procedures describing the handling of all written and oral complaints regarding a drug product are required. The quality control unit is responsible for reviewing any complaint involving the possible failure of a drug product to meet any of its speci cations and, for such drug products, making a determination as to the need for an investigation in accordance with cGMP requirements. The review should include a determination if the complaint represents a serious and unexpected adverse drug experience, which is required to be reported to the FDA. A written record of each complaint must be maintained in a complaint le.

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CHROMATOGRAPHY Hengen, P.N. (1995) Methods and reagents puri cation of Histag fusion proteins from Escherichia coli. Trends in Biochemical Sciences, 20, 285 6. Description of use of His-tag af nity puri cation. Kawasaki, T. (1991) Hydroxyapatite as a liquid chromatographic packing. Journal of Chromatography A, 544, 147 84. Review of hydroxyapatite in chromatography focusing on mode of interaction with sample components. Ladiwala A., Rege, K., Breneman, C.M. and Cramer, S.M. (2005) A priori prediction of adsorption isotherm parameters and chromatographic behavior in ion exchange systems. Proceeding of the National Academy of Sciences, USA, 102, 11710 15. Consideration of factors critical to protein behaviour in ion exchange chromatography. Laing, G.D., Kamiguti, A., Wilkinson, M.C. et al. (1995) Characterization of a puri ed phospholipase A2 from the venom of the papuan black snake (Pseudechis papuanus). Biochimica et Biophysica Acta, 1250, 137 43. An excellent example of application of hydrophobic interaction chromatography. Lienqueo, M.E. and Mahn, A. (2005). Predicting protein retention time in hydrophobic interaction chromatography. Chemical Engineering and Technology, 28, 1326 34. A review on hydrophobic interaction chromatography. Regnier, F.E. (1991) Perfusion chromatography. Nature, 350, 634 5. An overview of perfusion chromatography. Sheehan, D. (2008) Ion exchange chromatography, in Molecular Biomethods Handbook (eds J. Walker and R. Rapley), 2nd edn, Humana Press, Totowa, NJ, USA. Description of protein ion exchange chromatography. Winzor, D.J. (2003) Exclusion chromatography. Journal of Biochemical and Biophysical Methods, 56, 15 52. A review of the origin and uses of size exclusion (gel ltration) chromatography.

Figure 5-4: Dragging with Photoshop s edit tools creates these effects. The boundaries of each line are highlighted so that you can clearly see the distinctions between line and background.

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